Journal: Frontiers in Veterinary Science

Article Title: The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis

doi: 10.3389/fvets.2020.609443

Figure Lengend Snippet: The increase of TGF-β1 concentrations in blood plasma of M. bovis -infected cattle. (A) TGF-β1 concentrations in the plasma from M. bovis -infected ( n = 22) and -uninfected ( n = 16) cattle were determined by ELISA. Statistical significance was determined by the Mann-Whitney U test. (B) The correlation between TGF-β1 and PGE 2 concentrations in the plasma of M. bovis -infected cattle ( n = 19). Correlation statistic was analyzed using the Spearman correlation.

Article Snippet: In accordance with manufacturer's protocols, recombinant human TGF-β1 was reconstituted in sterile 4 mM HCl (Kanto Chemical, Tokyo, Japan), and 4 mM HCl was used as a vehicle control in the experiments.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Frontiers in Veterinary Science

Article Title: The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis

doi: 10.3389/fvets.2020.609443

Figure Lengend Snippet: The induction of TGF-β1 production by M. bovis . PBMCs from uninfected cattle were cultured with live M. bovis , and TGF-β1 concentrations in culture supernatants were measured by ELISA ( n = 8). Data are presented as means, and the error bars indicate standard errors. Statistical significance was determined by the Wilcoxon signed-rank test. MOI, multiplicity of infection.

Article Snippet: In accordance with manufacturer's protocols, recombinant human TGF-β1 was reconstituted in sterile 4 mM HCl (Kanto Chemical, Tokyo, Japan), and 4 mM HCl was used as a vehicle control in the experiments.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Infection

Journal: Frontiers in Veterinary Science

Article Title: The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis

doi: 10.3389/fvets.2020.609443

Figure Lengend Snippet: The effect of TGF-β1 on CD69 expression in cattle. (A–D) PBMCs from uninfected cattle ( n = 7) were incubated with TGF-β1 in the presence of Con A. (A) The gating strategy and representative plots for CD69 expression. (B–D) CD69 expression in T cells (B) , CD4 + T cells (C) , and CD8 + T cells (D) were measured by flow cytometry. Data are presented as means, and the error bars indicate standard errors. Statistical significance was determined by the Wilcoxon signed-rank test.

Article Snippet: In accordance with manufacturer's protocols, recombinant human TGF-β1 was reconstituted in sterile 4 mM HCl (Kanto Chemical, Tokyo, Japan), and 4 mM HCl was used as a vehicle control in the experiments.

Techniques: Expressing, Incubation, Flow Cytometry

Journal: Frontiers in Veterinary Science

Article Title: The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis

doi: 10.3389/fvets.2020.609443

Figure Lengend Snippet: The effect of TGF-β1 on cytokine production in cattle. (A–C) PBMCs from uninfected cattle ( n = 7) were incubated with TGF-β1 in the presence of Con A. After incubation, IFN-γ (A) , TNF-α (B) , and IL-10 (C) concentrations in culture supernatants were determined by ELISA. Data are presented as means, and the error bars indicate standard errors. Statistical significance was determined by the Wilcoxon signed-rank test.

Article Snippet: In accordance with manufacturer's protocols, recombinant human TGF-β1 was reconstituted in sterile 4 mM HCl (Kanto Chemical, Tokyo, Japan), and 4 mM HCl was used as a vehicle control in the experiments.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay